HRM Fast PCR Kits

Engineered for fast and accurate detection of DNA sequence variations.

High Resolution Melt (HRM) analysis is a technique for fast, high-throughput post-PCR analysis of genetic mutations or variance in nucleic acid sequences. The KAPA HRM FAST PCR Kit contains a convenient, ready-to-use master mix designed for the high performance detection of DNA sequence variations.

The KAPA HRM FAST Master Mix contains a novel DNA polymerase and buffer system, and EvaGreen® saturating dye optimized for maximum discrimination between sequence variants. Kits are ideally suited for:

  • SNP genotyping
  • Mutation discovery (gene scanning)
  • Screening for heterozygosity
  • DNA fingerprinting
  • DNA methylation analysis

 

CodeDescriptionKit Contents  
KK4201   KAPA HRM FAST PCR Kit (1 mL) For 100 x 20 µL reactions. Supplied with separate 1 x 1.6 mL 25 mM MgCl2. l
KK4202   KAPA HRM FAST PCR Kit (5 mL) For 500 x 20 µL reactions. Supplied with separate 2 x 1.6 mL 25 mM MgCl2.  
KK4203   KAPA HRM FAST PCR Kit (2 x 5 mL) For 1000 x 20 µL reactions. Supplied with separate 4 x 1.6 mL 25 mM MgCl2.

  • kapa2gFast.jpeg
    KAPA2G Fast PCR Kits A second-generation DNA polymerase engineered for the ultimate in speed and performance. KAPA2G Fast DNA Polymerase is a second-generation enzyme derived throu...

  • WEB_2G_Fast_image_v1.08.jpg
    KAPA2G Fast Multiplex PCR Kits High speed, high performance multiplex PCR without the need for optimization. KAPA2G Fast Multiplex PCR Kits contain a second-generation (2G) enzym...

  • robust-ready-mix.jpg
    KAPA2G Robust PCR Kits Streamline your PCR workflows by consolidating protocols and reaction conditions, increase pass rates, amplify difficult templates without bias, and increase...

  • KAPA_LONG_RANGE.jpg
    KAPA Long Range PCR Kits Amplify long templates up to 20 kb with extreme sensitivity and increased fidelity. The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an ...

  • banner SYBR.png
    KAPA SYBR® FAST qPCR Kits Containing the first DNA polymerase evolved for real-time PCR. KAPA SYBR® FAST qPCR Kits are designed for the highest performance in real-time PCR. Kits c...

  • kapasyrbfastonesteqrt.png
    KAPA SYBR® FAST One-Step qRT-PCR Kits Containing the first DNA polymerase evolved for real-time PCR. KAPA SYBR® FAST One-Step qRT-PCR Kits contain M-MuLV Reverse Transcript...

  • Probe.jpg
    KAPA PROBE FAST qPCR Kits Precise, reproducible, and versatile for all probe-based qPCR applications. KAPA PROBE FAST qPCR Kits contain a ready-to-use master mix for highly sensiti...

  • 3g-plantbanner.jpg
    KAPA3G Plant PCR Kits Evolved to solve. Direct PCR from plant tissue. The KAPA3G Plant PCR Kit is based on a novel, third-generation (3G) DNA polymerase, engineered via molecular e...

  • Kapa_box.jpg
    KAPA T4 DNA Ligase KAPA T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5’ phosphate and 3’ hydroxyl termini in duplex DNA or RNA. This enzyme will join blun...
Visitors: 213,024