Visualize a specific RNA locus at single cell sensitivity with spatial and morphological context
The BaseScopeTM Assay is a unique product from ACD, based on the same platform of p...
RNAscope Assays
Four flexible solutions for all your research needs
Independent of your genes of interest, our easy RNA in situ hybridization assay uses common reagents and protocols; providing universal assay conditions. All RNA-ISH kits contain reagents in a convenient ready-to-use (RTU) format, and chromogenic dyes are available to interrogate a single or two genes simultaneously. Most versatile are our RNAscope® Multiplex Fluorescent Reagent Kits, enabling you to interrogate 1, 2 or 3 genes simultaneously.
Automated RNAscope® ISH assays are also available for Leica Systems (LS) and Ventana Systems (VS).
Which RNAscope® Assay is right for me?
Use the table below to learn more about which RNA in situ hybridization product is right to your research needs.
|
Assay Type |
Chromogenic |
Chromogenic |
Chromogenic |
Fluorescent |
|
Dye Used |
Diaminobenzidine (DAB) |
Fast Red |
HRP Green & Fast Red |
TSA-based Opal fluorophores |
|
RNAscope® Probes Channel Designation |
Channel 1 |
Channel 1 |
Channel 1 & 2 |
Channel 1-4 |
|
Multiplexing |
Singleplex |
Singleplex |
Singleplex, Duplex |
Single to Fourplex |
|
Key Benefit |
Robust, sensitive, permanent stain. Most widely used RNAscope assay |
Provides bright color stains with high contrast to background |
Utilizes 2 different staining enzymes therefore avoiding any cross talk between the two stains |
Utilizes upto 4 different spectral channels providing high flexibility |
|
Ideal for |
First time user Routine applications |
Studies of tissues with endogenous color background such as melanin in skin, liver, or lungs from smokers |
Studies interrogating two RNA biomarkers simultaneously |
Co-localization studies of upto 4 genes simultaneously Experimental application requiring flexibility |
|
Novel Gene or Unknown Expression |
Compatible |
Compatible |
Compatible |
Compatible |
|
Archival Specimens |
Compatible |
Compatible |
Compatible |
Compatible |
|
Microscope Imaging System |
Standard bright field |
Standard bright field Multispectral fluorescent imaging |
Standard bright field |
Multispectral fluorescent imaging |
|
Sample Type* |
FFPE Tissues(TMAs) |
FFPE Tissues(TMAs) |
FFPE Tissues(TMAs) |
FFPE Tissues(TMAs) |
*Formalin-fixed, paraffin-embedded (FFPE), Tissue-Microarray (TMA)
