Fast PCR
KAPA2G Fast PCR Kits
A second-generation DNA polymerase engineered for the ultimate in speed and performance.
KAPA2G Fast DNA Polymerase is a second-generation enzyme derived through a process of molecular evolution. The KAPA2G Fast DNA Polymerase was engineered for higher processivity and speed, offering significantly faster extension rates and improved PCR success rates than wild-type Taq polymerase. KAPA2G Fast is well suited for both routine and high-throughput PCR, achieving higher yields and improved sensitivity than competitor enzymes across a wide range of amplicon types.
KAPA2G Fast DNA Polymerase and optimized buffer system offers:
- Extension times as low as 1 sec per kilobase.
- Outstanding performance when compared to standard and hot start Taq polymerase.
- 20 - 70% reductions in total PCR time.
- No requirement for specialist PCR consumables or instrumentation.
KAPA2G Fast DNA Polymerase is also available with HotStart technology for improved specificity during prolonged benchtop setup or liquid handling.
Code | Description | Kit Contents | ||
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KAPA2G Fast DNA Polymerase | ||||
KK5008 | KAPA2G Fast DNA Polymerase with dNTPs (100 U) |
100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).
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KK5009 | KAPA2G Fast DNA Polymerase with dNTPs (250 U) |
250 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).
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KK5020 | KAPA2G Fast DNA Polymerase (100 U) |
100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).
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KK5021 | KAPA2G Fast DNA Polymerase (250 U) |
250 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).
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KK5101 | KAPA2G Fast ReadyMix with dye (100 x 25 µL reactions) |
Kit includes 100 x 25 µl reactions of KAPA2G Fast ReadyMix with dye, a convenient 2X PCR master mix containing KAPA dNTPs, KAPA2G Fast DNA Polymerase, reaction buffer, Mg2+, and loading dye.
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KK5102 | KAPA2G Fast ReadyMix with dye (500 x 25 µL reactions) |
Kit includes 500 x 25 µl reactions of KAPA2G Fast ReadyMix with dye, a convenient 2X PCR master mix containing KAPA dNTPs, KAPA2G Fast DNA Polymerase, reaction buffer, Mg2+, and loading dye.
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KAPA2G Fast HotStart DNA Polymerase | ||||
KK5500 | KAPA2G Fast HotStart DNA Polymerase with dNTPs (500 U) |
500 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).
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KK5501 | KAPA2G Fast HotStart DNA Polymerase (500 U) |
500 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).
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KK5502 | KAPA2G Fast HotStart DNA Polymerase with dNTPs (250 U) |
250 U, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).
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KK5503 | KAPA2G Fast HotStart DNA Polymerase (250 U) |
250 units, 5 U/µL. Supplied with proprietary 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).
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KK5510 | KAPA2G Fast HotStart DNA Polymerase with dNTPs - Mg2+ free buffer (500 U) |
500 units, 5 U/µL. Supplied with Mg2+ free 5X KAPA2G Buffer M, dNTP Mix (10 mM) and MgCl2 (25 mM).
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KK5511 | KAPA2G Fast HotStart DNA Polymerase - Mg2+ free buffer (500 U) |
500 units, 5 U/µL. Supplied with Mg2+ free 5X KAPA2G Buffer M and MgCl2 (25 mM).
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KK5512 | KAPA2G Fast HotStart DNA Polymerase with dNTPs - Mg2+ free buffer (250 U) |
250 units, 5 U/µL. Supplied with Mg2+ free 5X KAPA2G Buffer M, dNTP Mix (10 mM) and MgCl2 (25 mM).
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KK5513 | KAPA2G Fast HotStart DNA Polymerase - Mg2+ free buffer (250 U) |
KAPA2G Fast HotStart DNA Polymerase.^(250 U, 5 U/µL). With Mg-free proprietary 5X KAPA2G Buffer M and MgCl2 (25 mM).
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KK5523 | KAPA2G Fast HotStart DNA Polymerase (100 U) |
100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).
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KK5530 | KAPA2G Fast HotStart DNA Polymerase with dNTPs (100 U) |
100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).
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KK5601 | KAPA2G Fast HotStart ReadyMix (500 x 25 µL rxns) |
KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM.
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KK5603 | KAPA2G Fast HotStart ReadyMix (100 x 25 µL rxns) |
KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM.
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KK5608 | KAPA2G Fast HotStart ReadyMix with dye (100 x 25 µL reactions) |
KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format with loading dye. Contains Mg2+ at a 1X conc. of 1.5 mM.
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KK5609 | KAPA2G Fast HotStart ReadyMix with dye (500 x 25 µL reactions) |
KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format with loading dye. Contains Mg2+ at a 1X conc. of 1.5 mM.
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KAPA2G Fast Multiplex PCR Kits High speed, high performance multiplex PCR without the need for optimization. KAPA2G Fast Multiplex PCR Kits contain a second-generation (2G) enzym...
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KAPA2G Robust PCR Kits Streamline your PCR workflows by consolidating protocols and reaction conditions, increase pass rates, amplify difficult templates without bias, and increase...
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KAPA Long Range PCR Kits Amplify long templates up to 20 kb with extreme sensitivity and increased fidelity. The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an ...
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KAPA SYBR® FAST qPCR Kits Containing the first DNA polymerase evolved for real-time PCR. KAPA SYBR® FAST qPCR Kits are designed for the highest performance in real-time PCR. Kits c...
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KAPA SYBR® FAST One-Step qRT-PCR Kits Containing the first DNA polymerase evolved for real-time PCR. KAPA SYBR® FAST One-Step qRT-PCR Kits contain M-MuLV Reverse Transcript...
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KAPA PROBE FAST qPCR Kits Precise, reproducible, and versatile for all probe-based qPCR applications. KAPA PROBE FAST qPCR Kits contain a ready-to-use master mix for highly sensiti...
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Engineered for fast and accurate detection of DNA sequence variations. High Resolution Melt (HRM) analysis is a technique for fast, high-throughput post-PCR analysis of genetic mut...
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KAPA3G Plant PCR Kits Evolved to solve. Direct PCR from plant tissue. The KAPA3G Plant PCR Kit is based on a novel, third-generation (3G) DNA polymerase, engineered via molecular e...
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KAPA T4 DNA Ligase KAPA T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5’ phosphate and 3’ hydroxyl termini in duplex DNA or RNA. This enzyme will join blun...