SYBR Green qPCR Kits

KAPA SYBR® FAST qPCR Kits

Containing the first DNA polymerase evolved for real-time PCR.

KAPA SYBR® FAST qPCR Kits are designed for the highest performance in real-time PCR. Kits contain a novel DNA polymerase engineered through a process of molecular evolution - the result is a unique enzyme, specifically evolved for qPCR using SYBR® Green I dye chemistry.

The KAPA SYBR® DNA Polymerase was engineered to perform optimally in stringent real-time qPCR reaction conditions, exhibiting dramatic improvements to signal-to-noise ratio (fluorescence), cycle threshold (Ct), linearity, and sensitivity. The KAPA SYBR® DNA Polymerase and proprietary buffer system enhances the amplification efficiency of difficult templates, including both GC-rich and AT-rich templates.

 

CodeDescriptionKit Contents  
KK4600 KAPA SYBR® FAST Universal 2X qPCR Master Mix (1 mL) For 100 x 20 µL reactions. Supplied with separate 50X ROX High and 50X ROX Low (200 µL each).  
KK4603 KAPA SYBR® FAST ABI Prism® 2X qPCR Master Mix (1 mL) For 100 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® PRISM®7000, 7700, 7900HT, ABI 7300 Real-Time PCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™.  
KK4606 KAPA SYBR® FAST Bio-Rad iCycler 2X qPCR Master MIx (1 mL) For 100 x 20 µL reactions. Fluorescein is included in the master mix at an optimal concentration for most Bio-Rad qPCR cyclers.  
KK4609 KAPA SYBR® FAST Roche LightCycler® 480 2X qPCR Master Mix (1 mL) For 100 x 20 µL reactions. Optimized for use with the Roche LightCycler® 480.  
KK4619 KAPA SYBR® FAST ROX Low 2X qPCR Master Mix (1 x 1 mL) For 100 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® 7500, ViiA™ 7, QuantStudio™ 12K Flex, Agilent Mx3000P™, Mx3005P™ and Mx4000™.  

 

 


  • kapa2gFast.jpeg
    KAPA2G Fast PCR Kits A second-generation DNA polymerase engineered for the ultimate in speed and performance. KAPA2G Fast DNA Polymerase is a second-generation enzyme derived throu...

  • WEB_2G_Fast_image_v1.08.jpg
    KAPA2G Fast Multiplex PCR Kits High speed, high performance multiplex PCR without the need for optimization. KAPA2G Fast Multiplex PCR Kits contain a second-generation (2G) enzym...

  • robust-ready-mix.jpg
    KAPA2G Robust PCR Kits Streamline your PCR workflows by consolidating protocols and reaction conditions, increase pass rates, amplify difficult templates without bias, and increase...

  • KAPA_LONG_RANGE.jpg
    KAPA Long Range PCR Kits Amplify long templates up to 20 kb with extreme sensitivity and increased fidelity. The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an ...

  • kapasyrbfastonesteqrt.png
    KAPA SYBR® FAST One-Step qRT-PCR Kits Containing the first DNA polymerase evolved for real-time PCR. KAPA SYBR® FAST One-Step qRT-PCR Kits contain M-MuLV Reverse Transcript...

  • Probe.jpg
    KAPA PROBE FAST qPCR Kits Precise, reproducible, and versatile for all probe-based qPCR applications. KAPA PROBE FAST qPCR Kits contain a ready-to-use master mix for highly sensiti...

  • HRM.jpg
    Engineered for fast and accurate detection of DNA sequence variations. High Resolution Melt (HRM) analysis is a technique for fast, high-throughput post-PCR analysis of genetic mut...

  • 3g-plantbanner.jpg
    KAPA3G Plant PCR Kits Evolved to solve. Direct PCR from plant tissue. The KAPA3G Plant PCR Kit is based on a novel, third-generation (3G) DNA polymerase, engineered via molecular e...

  • Kapa_box.jpg
    KAPA T4 DNA Ligase KAPA T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5’ phosphate and 3’ hydroxyl termini in duplex DNA or RNA. This enzyme will join blun...
Visitors: 212,447