SYBR Green qPCR Kits

KAPA SYBR® FAST qPCR Kits

Containing the first DNA polymerase evolved for real-time PCR.

KAPA SYBR® FAST qPCR Kits are designed for the highest performance in real-time PCR. Kits contain a novel DNA polymerase engineered through a process of molecular evolution - the result is a unique enzyme, specifically evolved for qPCR using SYBR® Green I dye chemistry.

The KAPA SYBR® DNA Polymerase was engineered to perform optimally in stringent real-time qPCR reaction conditions, exhibiting dramatic improvements to signal-to-noise ratio (fluorescence), cycle threshold (Ct), linearity, and sensitivity. The KAPA SYBR® DNA Polymerase and proprietary buffer system enhances the amplification efficiency of difficult templates, including both GC-rich and AT-rich templates.

 

CodeDescriptionKit Contents  
KK4600 KAPA SYBR® FAST Universal 2X qPCR Master Mix (1 mL) For 100 x 20 µL reactions. Supplied with separate 50X ROX High and 50X ROX Low (200 µL each).  
KK4603 KAPA SYBR® FAST ABI Prism® 2X qPCR Master Mix (1 mL) For 100 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® PRISM®7000, 7700, 7900HT, ABI 7300 Real-Time PCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™.  
KK4606 KAPA SYBR® FAST Bio-Rad iCycler 2X qPCR Master MIx (1 mL) For 100 x 20 µL reactions. Fluorescein is included in the master mix at an optimal concentration for most Bio-Rad qPCR cyclers.  
KK4609 KAPA SYBR® FAST Roche LightCycler® 480 2X qPCR Master Mix (1 mL) For 100 x 20 µL reactions. Optimized for use with the Roche LightCycler® 480.  
KK4619 KAPA SYBR® FAST ROX Low 2X qPCR Master Mix (1 x 1 mL) For 100 x 20 µL reactions. ROX is included in the master mix at an optimal concentration for use with the following instruments: Applied Biosystems® 7500, ViiA™ 7, QuantStudio™ 12K Flex, Agilent Mx3000P™, Mx3005P™ and Mx4000™.  

 

 


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