nCounter Technology

The nCounter Analysis System utilizes a novel digital color-coded barcode technology that is based on direct multiplexed measurement of gene expression and offers high levels of precision and sensitivity (>1 copy per cell). The technology uses molecular "barcodes" and single molecule imaging to detect and count hundreds of unique transcripts in a single reaction.

Each color-coded barcode is attached to a single target-specific probe corresponding to a gene of interest. Mixed together with controls, they form a multiplexed CodeSet.

• Multiplex hundreds of gene targets in a single reaction
• High sensitivity (< 1 copy per cell)
• Fully-automated system
• Exceptionally easy-to-use
• No enzymes required to perform assay
• Multiplex 800 regions from as little as 300ng of DNA
• 10 invariant region controls included in every CodeSet

      Molecules That Count®
Each color-coded barcode represents a single target molecule. Barcodes hybridize directly to your target molecules and can be individually counted without the need for amplification – providing very sensitive digital data.

• NanoString’s Technology employs two ~50 base probes per mRNA that hybridize in solution. The Reporter Probe carries the signal; the Capture Probe allows the complex to be immobilized for data collection.

  
  

• After hybridization, the excess probes are removed and the probe/target complexes aligned and immobilized in the nCounter Cartridge

  
  

• Sample Cartridges are placed in the Digital Analyzer for data collection. Color codes on the surface of the cartridge are counted and tabulated for each target molecule.