Designed to identify the most meaningful variants across a variety of solid tumor types, this panel includes somatic variants (SNVs, DNVs, and small InDels) associated with lung ad...
Vantage 3D™ DNA SNV Assays
Simple, digital detection of single nucleotide variants (SNVs) and small InDels from as little as 5 ng of DNA from FFPE in a single tube. Designed for compatibility with other 3D Biology™ Assays, Vantage 3D DNA SNV Assays deliver reliable SNV detection that may be combined with mRNA, gene fusion, and protein for in-depth characterization of key oncogenesis pathways.
- Maximum information from only 5 ng of input DNA for SNV and InDel detection
- Simple protocol, optimized for challenging FFPE samples
- Validated workflows for cell suspensions, fresh/frozen tissue, and FFPE
- Concordance with gold standard mutational analysis techniques
- Sensitivity and Specificity above 95% on solid tumor samples with >5% allele frequency*
- Customizable and forward-compatible with our growing line of 3D Biology Products
- Simple, integrated data analysis, eliminating the need for a bioinformatician
*Validated on internal reference materials. Data may vary with the various quality of FFPE samples.
The nCounter® Vantage 3D DNA SNV Assays leverage NanoString’s proven molecular barcode technology. Each SNV panel is designed to detect the mutant allele of interest and a reference allele using a set of probes with novel architecture. The upstream probe (Probe S) has proprietary sequences that become unstable in the presence of a single nucleotide mismatch. The downstream probe (Probe T) is based on existing NanoString probe chemistry. Pools of Probe S and T are combined with an nCounter® molecular barcode SNV TagSet to enable highly specific detection of SNVs in a background of abundant normal tissue alleles.
