Get accurate results quickly from serum, plasma, and FFPE with nCounter® miRNA Assays. The assays allow you to produce highly robust biological information from low sample inputs i...
miRGE™ Assay
Simultaneously detect subsets of mRNA and miRNA in total RNA for biomarker discovery. The nCounter® miRGE assay is uniquely designed to provide a single-tube, sensitive, reproducible, and highly multiplexed method.
- Choose custom 5–30 miRNAs and 100–200 mRNAs for simultaneous analysis
- Available for human, mouse, and rat
- Compatible with total RNA from serum, plasma, FFPE, tissue, cells, and exosomes
- No reverse transcription, no amplification, and fewer pipetting steps means less chance for errors and bias in results
| Feature | Specifications |
| Level of Multiplexing | 100-200 mRNAs 5-30 miRNAs |
| Recommended amount of starting material | 100 ng of purified total RNA |
| Sample types supported | Purified total RNA |
| Sample prep reaction volume | 10 μL |
| Hybridization reaction volume | 35 μL |
| Limit of Detection: miRNA | > 2.5 copies per cell copy per cell |
| Limit of Detection: mRNA | 1 copy per cell |
| Fold-change sensitivity | > 2-fold change |
| Hybridization Spike Correlation | R² ≥ 0.95 |
| Linear Dynamic Range | 2 x 10⁶ total counts |
| nCounter® Prep Station throughput | 12 samples < 2.5 hours |
| nCounter® Digital Analyzer throughput | 12 samples/4 hours (up to 72 samples per day) unmattended running in continuous mode) |
| Controls | 6 positive hybridization controls 8 negative hybridization controls 2 positive ligation controls |
| Unit Size | 48, 96, 192 assays |
| Shipping Conditions | -80°C |
