Vantage 3D™ Immuno-Oncology Assays

Quantify protein and gene expression in one experiment and gain deeper insight into your immuno-oncology research. Designed with protein content matched to the gene expression 770-plex PanCancer Immune Profiling Panel for characterization of up to 800 total targets, these assays enable more data from less sample.

Detect 770 mRNA and up to 30 proteins from as few as 20,000 cells
Content designed to profile immune cell type and functional state
Compatible with PBMC and other primary cells
Simple integrated workflow and holistic data output
Easy data management: No bioinformatician required

Applications

Compatible with 3D Flow™ Analysis

Post-translational Regulation

NanoString data demonstrates that combined RNA and protein profiling reveals potential post-transcriptional regulation for a subset of targets and protein expression data that is highly correlated with gold standard flow cytometry.

PBMC were stimulated with PMA and RNA and protein expression was profiled using the nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay (input of 50,000 PBMC). Additionally, a subset of protein targets were analyzed by flow cytometry and data from both platforms showed high correlation.

Characterizing Macrophage Differentiation and Polarization

Integrated analysis enables simultaneous quantitation of RNA and protein expression from a single sample and eliminates the need to compare data from different technologies with different outputs.

A THP-1 monocytic cell line was induced to differentiate into a macrophage phenotype with PMA. Cells were then polarized to an M1 or M2 phenotype by 24h culture using IFNγ + LPS, or IL4, respectively. Gene and protein expression was characterized at each stage of differentiation using the nCounter Vantage 3D RNA:Protein Immune Cell Profiling Assay.

Precise Cell Profiling

RNA:Protein signatures can accurately identify specific immune cell types, allowing you to uncover changes in a heterogeneous cell population in response to external stimuli, such as immunotherapeutic agents, for example.

Vantage 3D™ Heme Assays

Profile changes in DNA, RNA, and protein expression in a single experiment. The Vantage 3D DNA:RNA:Protein Heme Assays are designed to provide a complete heme profiling solution fo...

Vantage 3D™ Solid Tumor Assays

Profile changes in DNA, RNA, and protein expression in a single experiment. The Vantage 3D DNA:RNA:Protein Solid Tumor Assays are designed to provide a complete solid tumor profili...

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