Blotting

Detection Reagents

Before membrane bound antigens can be detected, unspecific binding sites for antibodies on the transfer membrane have to be blocked. For many applications skim milk powder is an economic alternative to bovine serum albumin (BSA). For Western Blots where skim milk cannot be used, SERVA offers highly purified BSA like Albumin bovine Fraction V, pH 7.0, cat. no. 11930.
After blotting of nucleic acids before hybridization, non-specific binding sites are blocked by Denhardt’s solution (cat. no. 39603). For preparation of Denhardt’s solution or other molecular biology applications, Albumin bovine DNAse/RNase free, cat no. 11967 is the optimal choice.
In addition to a broad range of high quality substrates as powder, SERVA offers ready-to-use, highly sensitive substrate solutions for easy and fast detection of proteins in Western Blots.
SERVALight HRP Chemiluminescence Kits are a new family of highly sensitive ready-to-use kits for chemiluminescence detection of membrane bound antigens (Western Blot) or nucleic acid sequences (Southern and Northern Blot), labelled directly with Horseradish Peroxidase (HRP) or indirectly with HRP-conjugated antibodies/streptavidin. They are easy to use, have an excellent stability, extended signal duration and save money and precious antibodies due to high dilution of antibodies.