Description 
The FavorPrep™ Plasmid Extraction Kit provides a rapid, phenol-free method for the extraction of high-purity plasmid DNA from bacterial cultures such as E. coli , which bacteria is pellet, lysed, and then neutralized. The extracted DNA can be used in a variety of applications such as PCR, cloning, sequencing, in vitro transcription, and labeling. Also, as a column-type tube is utilized in the purification process, extraction is carried out in three simple steps of binding / washing / elution. Once bound, the DNA is washed and then eluted from the column, ready for use.
Features
•  For high yields of plasmid DNA-up to 30 μg from 1~5 ml overnight cultures.
•  No need for messy resin slurries, extracting with phenol, or concentrating via alcohol precipitation.
•  Superior purity-DNA yields quality sequence data using automated or manual methods.
•  Optimized buffers are included for maximum DNA purity and yield.
•  Versatile protocol-works with all neutral gel buffers and both conventional and low-melt agarose gel.

The FavorPrep™ Plasmid Extraction Midi and Maxi Kit provides pre-packed an ion exchange resin column for high-purity plasmid DNA maxiperp from 50 - 300 ml bacterial cultures. Depending on the copy-number of plasmids, either a 50-150ml (low-copy plasmid) or a 150 - 500 ml (high-copy number) bacterial suspension could be used in the process. When using the standard protocol, the entire midi prep process can be completed in 120 minutes or less and up to 500 μg of plasmid can be expected. The purity of purified plasmid DNA is equity to that obtained by 2x CsCl-gradient centrifugation and suitable for all molecular biological applications.
Features
•  Purity: equity to that obtained by 2x CsCl-gradient centrifugation . 
•  Safe: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, minimizing exposure to, and disposal of hazardous materials.
•  Time saving: Complete the process in less than 120 minutes.

The FavorFilter Plasmid DNA Extraction Maxi Kit is designed for rapid and efficient extraction of high quality plasmid DNA. With provided filter cartridges the bacteria lysates will be removed without centrifugation. Following a gravity-flow procedure, the plasmid DNA is bound to the resin, and the contaminants can be remove with wash buffer. After using this convenient kit, the purified plasmid DNA is suitable for downstream application such as transfection, in vitro transcription and translation, and all enzymatic modification.

Features

•  Time saving: Cleared lysate without centrifugation
•  DNA Yield and Sample Size
•  up to 500μg 
100~250 cultured volume
•  High Purity: Equal to that obtained by 2x CsCl gradient centrifugation.
•  Safe: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, minimizing exposure to and disposal of hazardous materials.

Applications 
The purified plasmid DNA can be used immediately in downstream application.
•  Transfection (non-endotoxin sensitive)
•  Microinjection
•  In Virto transcription
•  Restriction Enzyme digestion

Procedure 
In the process, after the modified alkaline lysis, the filter cartridge is used to remove bacteria lysates to obtain cleared sample matrix. Then the plasmid DNA will bind to the onion exchange resin inside the Favorgen Maxi Column when the sample matrix flowing through. The contaminants can be removed by wash buffer. Finally, the purified plasmid DNA is eluted using high-salt buffer and then precipitated with iospropanol for desalting.

Cat. No.	Product Name	Size	Kit Contents	Store at	Protocol
FAPDE 001	FavorPrep™ Plasmid DNA Extraction Mini Kit	100 preps.	FAPD 1 Buffer  FAPD 2 Buffer  FAPD3 Buffer  W1 Buffer Wash Buffer (conc.)  Elution Buffer  RNase A (50 mg/ml)  FAPD Columns  2 ml Collection Tube	FAPD 1 Buffer with RNaseA at 4 ℃ , other components should at room temperature (15 -25 ℃ ).
FAPDE 001-1	FavorPrep™ Plasmid DNA Extraction Mini Kit	300 preps.	FAPD 1 Buffer  FAPD 2 Buffer  FAPD3 Buffer  W1 Buffer Wash Buffer (conc.)  Elution Buffer  RNase A (50 mg/ml)  FAPD Columns  2 ml Collection Tube	FAPD 1 Buffer with RNaseA at 4 ℃ , other components should at room temperature (15 -25 ℃ ).

Cat. No.	Product Name	Size	Kit Contents	Store at	Protocol
FAPDE 002	FavorPrep™ Plasmid DNA Extraction Midi Kit	25 preps.	PM1 Buffer  PM2 Buffer  PM3 Buffer  PEQ Buffer  PW Buffer  PEL Buffer  RNase A (50mg/ml)  PM Midi columns	PM1 Buffer with RNaseA at 4 ℃ , others at room temperature.
FAPDE 002-1	FavorPrep™ Plasmid DNA Extraction Midi Kit	50 preps.	PM1 Buffer  PM2 Buffer  PM3 Buffer  PEQ Buffer  PW Buffer  PEL Buffer  RNase A (50mg/ml)  PM Midi columns	PM1 Buffer with RNaseA at 4 ℃ , others at room temperature.
FAPDE 003	FavorPrep™ Plasmid DNA Extraction Maxi Kit	10 preps.	PM1 Buffer  PM2 Buffer  PM3 Buffer  PEQ Buffer  PW Buffer  PEL Buffer  RNase A (50mg/ml)  PM Maxi columns	PM1 Buffer with RNaseA at 4 ℃ , others at room temperature.
FAPDE 003-1	FavorPrep™ Plasmid DNA Extraction Maxi Kit	20 preps.	PM1 Buffer  PM2 Buffer  PM3 Buffer  PEQ Buffer  PW Buffer  PEL Buffer  RNase A (50mg/ml)  PM Maxi columns	PM1 Buffer with RNaseA at 4 ℃ , others at room temperature.
FAPDE 002-EF	FavorPrep™ Endotoxin Free Plasmid DNA Extraction Midi Kit	25 preps.	PM1 Buffer  PM2 Buffer  PM3 Buffer  PEQ Buffer  PW Buffer  PEL Buffer PRT Buffer  RNase A (50mg/ml)  PM Midi columns	PM1 Buffer with RNaseA at 4 ℃ , others at room temperature.
FAPDE 003-EF	FavorPrep™ Endotoxin Free Plasmid DNA Extraction Maxi Kit	10 preps.	PM1 Buffer  PM2 Buffer  PM3 Buffer  PEQ Buffer  PW Buffer  PEL Buffer PRT Buffer  RNase A (50mg/ml)  PM Maxi columns	PM1 Buffer with RNaseA at 4 ℃ , others at room temperature.

Cat. No.	Product Name	Size	Kit Contents	Store at	Protocol
FAFTE 001	FavorFilter™ Plasmid DNA Extraction Maxi Kit	4 preps.	PM1 Buffer PM2 Buffer PM3 Buffer  PEQ Buffer  PW Buffer  PE Buffer  RNase A (50mg/ml) Favorfilter Maxi Cartridges PM Maxi Columns	PM1 Buffer with RNaseA at 4 ℃ , others at room temperature (15 -25 ℃ ).
FAFTE 001-1	FavorFilter™ Plasmid DNA Extraction Maxi Kit	10 preps.	PM1 Buffer PM2 Buffer PM3 Buffer  PEQ Buffer  PW Buffer  PE Buffer  RNase A (50mg/ml) Favorfilter Maxi Cartridges PM Maxi Columns	PM1 Buffer with RNaseA at 4 ℃ , others at room temperature (15 -25 ℃ ).
FAFTE 001-EF	FavorFilter™ Endotoxin Free Plasmid DNA Extraction Maxi Kit	4 preps.	PM1 Buffer PM2 Buffer PM3 Buffer  PEQ Buffer  PW Buffer  PE Buffer PRT Buffer RNase A (50mg/ml) Favorfilter Maxi Cartridges PM Maxi Columns	PM1 Buffer with RNaseA at 4 ℃ , others at room temperature (15 -25 ℃ ).
FAFTE 001-1-EF	FavorFilter™ Endotoxin Free Plasmid DNA Extraction Maxi Kit	10 preps.	PM1 Buffer PM2 Buffer PM3 Buffer  PEQ Buffer  PW Buffer  PE Buffer PRT Buffer RNase A (50mg/ml) Favorfilter Maxi Cartridges PM Maxi Columns	PM1 Buffer with RNaseA at 4 ℃ , others at room temperature (15 -25 ℃ ).